Keio University Human Biology-Microbiome-Quantum Research Center (WPI-Bio2Q) will hold a seminar as follows.
This is an event for faculty, students, and staff of Keio University.

Protein–protein interactions are central to cellular function and are organized within the spatial architecture of tissues. In the brain, even neighbouring regions can exhibit distinct molecular environments, yet most interactome approaches average signals across tissue and miss these local differences. Here we introduce DMD-PhoxID, a spatial proximity labelling strategy that uses patterned light to map protein environments within defined anatomical subregions. We first demonstrate that light driven proximity labelling can be tightly confined to adjacent cerebellar layers, as validated by imaging and proteomics. Applying this approach to endogenous AMPA receptors in the hippocampus reveals that core interaction partners are shared, while their local organization differs across CA1, CA2/3, and the dentate gyrus. DMD-PhoxID therefore provides a simple framework for linking brain anatomy with molecular interaction networks directly in intact tissue.