Keio University Human Biology-Microbiome-Quantum Research Center (WPI-Bio2Q) held a seminar as follows.

16:00 -17:00, February 19, 2026

Venue: 6F Meeting Room, Center for Integrated Medical Research, Shinanomachi Campus, Keio University

Speaker:
Takeharu Mino, PhD
Postdoctoral Researcher
Department of Synthetic and Biological Chemistry,
Graduate School of Engineering
Kyoto University, Japan

Title: “DMD-PhoxID Enables Spatially Resolved Protein Interaction Mapping”

Abstract: Protein–protein interactions are central to cellular function and are organized within the spatial architecture of tissues. In the brain, even neighbouring regions can exhibit distinct molecular environments, yet most interactome approaches average signals across tissue and miss these local differences. Here we introduce DMD-PhoxID, a spatial proximity labelling strategy that uses patterned light to map protein environments within defined anatomical subregions. We first demonstrate that light driven proximity labelling can be tightly confined to adjacent cerebellar layers, as validated by imaging and proteomics. Applying this approach to endogenous AMPA receptors in the hippocampus reveals that core interaction partners are shared, while their local organization differs across CA1, CA2/3, and the dentate gyrus. DMD-PhoxID therefore provides a simple framework for linking brain anatomy with molecular interaction networks directly in intact tissue.